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KMID : 0903520050480030207
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 3 p.207 ~ p.211
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Optimization of Culture Condition for the Hydrocinnamic Acid Production from Bacillus subtilis IJ-31
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Joo Gil-Jae
Kim Young-Mok
Lee Oh-Seuk
Kim Joung-Woong
Kim Won-Chan
Song Kyung-Sik
Yoon Sung-Joon
Kim Jin-Ho
Rhee In-Koo
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Abstract
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The metabolites released from cultures of rhizosphere bacteria can inhibit plant growth. Bacillus subtilis IJ-31 inhibited plant growth by the production of hydrocinnamic acid (HCA). The production of HCA by plant-growth inhibiting rhizobacterium B. subtilis IJ-31 was optimized. 90.5 μg/ml of HCA was obtained under the condition of 1% rice bran as carbon source, 0.5% tryptone as nitrogen source, 0.1% ZnCl2 as metal source at 37oC for 60 h (pH 7.0). The optimal condition for the HCA production by B. subtilis IJ-31 in the jar fermenter was established using response surface methodology (RSM) of statistical analysis system(SAS) program. The production of HCA by B. subtilis IJ-31 in the jar fermenter culture reached 102.99 μg/ml when 2.24% soil extracts was added and agitation speed was 290 rpm under the same condition. And the experimental value of HCA production is 102.5 μg/ml in the same culture condition. The production of HCA by B. subtilis IJ-31 is higher as 12% than that from the flask culture.
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KEYWORD
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plant-growth inhibiting rhizobacterium, optimization of production, hydrocinnamic acid, Bacillus subtilis
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